It involves the demonstration of the role and importance of the target protein in a diseased condition (pathogenicity). An important outgrowth of the study of new drug targets is the recognition that many traditional targets, such as enzymes and receptors, are considerably more heterogeneous than previously thought.
It is accomplished by using ribozyme, knock-out or knock-in animal, small molecule which function as inhibitors/agonists, antagonists, and antibodies.
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