It refers to the quantitative study of protein expression between samples differing by some variables. The pattern of expression of the complete proteome or of its part between samples can be compared with this approach. It is useful in identifying disease-specific proteins using various techniques like two-dimensional gel electrophoresis, mass spectrometry, microarray, etc.
The quickest way to assign a new gene or cDNA a function is to carry out pair wise comparison with cDNA and EST sequences, and known gene sequences stored in databases using alignment tools like BLAST or FASTA.
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