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Scatchard Analysis Assignment Help

Scatchard Analysis

A radio-labeled ligand (a hormone), is added at several concentration to a fixed amount of receptors and the fraction of the hormone bound to receptor is determined by separation the receptor/hormone complex from free hormone.

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It is used to estimate the dissociation constant and the number of receptor binding sites in a given preparation.

The various techniques through which it is achieved are discussed in brief as follows:

  1. Spectroscopy
  2. Mass Spectrometry
  3. Chromatography
  4. Electrophoresis
  5. Isoelectric Focussing
  6. Microscopy
  7. Scatchard Analysis
  8. FRET
  9. Blotting

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