Securing Higher Grades Costing Your Pocket? Book Your Assignment at The Lowest Price Now!


Isoelectric Focussing Assignment Help And Online Tutoring

Isoelectric Focussing

This method is used to separate protein according to their iso-electric points. A stable pH gradient is established in the gel by the addition of ampholytes. A protein mixture is placed in a well on the gel. With an applied electric field, protein enters the gel and migrates until each reaches a pH equivalent to its pI. Iso-electric focusing and SDS electrophoresis are combined sequentially in a process called two-dimensional electrophoresis. It is a more sensitive method than either of the above mentioned electrophoresis; it separates protein of identical molecular weight differing in Pi or vice-versa.

Such as an example of mixtures which is used as gel are called Ampholine and Pharmalyte have mixtures of 600 to 700 different homologues of amphoteric compounds with a spectrum of isoelectric points between 3 and 10 form a pH gradient under the influence of the electric field. Their buffering capacities is very high at their isoelectric points. Their molecular weights below 1 kDalton and these are hydrophilic.

Isoelectric Assignment Help Order Now

The various techniques through which it is achieved are discussed in brief as follows:

  1. Spectroscopy
  2. Mass Spectrometry
  3. Chromatography
  4. Electrophoresis
  5. Isoelectric Focussing
  6. Microscopy
  7. Scatchard Analysis
  8. FRET
  9. Blotting

Email Based Assignment Help in Analytical Biotechnology

To Schedule a Analytical Biotechnology tutoring session Live chat
To submit Analytical Biotechnology assignment click here

Isoelectric Assignment Help | Isoelectric Homework Help | Isoelectric Online Help | Biochemical engineering | Biological engineering | Biological chemistry | Science and biotechnology | Application of Biotechnology | Applied biology | Organisms | Biology Research Lab | Enzymes | Online Tutoring | Biochemical engineering | Carbohydrates | Lipids | Proteins | Nucleic acids | DNA | Bioreactors | Bioprocessing | Applied biochemistry and biotechnology | Applied chemical technology | Genetics engineering | Biochemical assignment help | Biotechnology assignment help | Cell biology | Biochemical processes | Biochemical nutrition | Molecular biology

Assignment Help Features
Assignment Help Services
  • Assignment Help
  • Homework Help
  • Writing Help
  • Academic Writing Assistance
  • Editing Services
  • Plagiarism Checker Online
  • Proofreading
  • Research Writing Help
QR Code Assignment Help