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Vector

A vector is a DNA molecule that has the ability to replicate autonomously in an appropriate host cell and into which the DNA fragment to be cloned (called DNA insert) is integrated for cloning. Therefore, a vector must have an origin of replication (ori) that functions in the host cell. Any extra-chromosomal small genome, e.g., plasmid, phage or virus, may be used as a vector. Some of the common vectors are E.Coli Vectors, plasmid vectors, bacteriophage vectors, cosmid vectors, phagemid vectors, phasmid vectors, artificial chromosome vectors, shuttle vectors, yeast vectors.

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The main topics which are considered in Recombinant DNA Technology are discussed in brief as follows:

  1. Gene Cloning
  2. Vector
  3. Chemical Synthesis Of Gene
  4. Enzymatic Synthesis Of DNA
  5. Polymerase Chain Reaction

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