Chemical Synthesis Of Gene Assignment Help

Chemical Synthesis Of Gene

The chemical synthesis of a gene utilizes chemical reagents. There are three distinct methods, differing mainly in the strategy of protection of the phosphate residues namely phosphodiester approach, phosphotriester approach and phosphoramidite approach.

Both the phosphotriester and phosphoramidite methods utilize deoxyribonucleosides as starting materials, and involve the stepwise addition of mononucleotides and oligonucleotides.

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The main topics which are considered in Recombinant DNA Technology are discussed in brief as follows:

  1. Gene Cloning
  2. Vector
  3. Chemical Synthesis Of Gene
  4. Enzymatic Synthesis Of DNA
  5. Polymerase Chain Reaction

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