Gene cloning involves the production and isolation of the DNA fragments to be cloned, its insertion into a suitable vector to obtain recombinant DNA, further introduction of the recombinant DNA into a suitable organism (the host) and then the selection of the transformed host cells which contain the desired gene/DNA fragment. The gene is then multiplied and expressed into the host.
Gene cloning utilizes certain biological products and biological agents for achieving its objectives. Four different types of enzymes are also used which are nucleases, ligases, polymerases, and DNA modifying enzymes.
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