Defined as the estimation of potency of an active principle in the unit quantity of the preparation. There are three categories of an assay:
Chemical methods are used for estimating the constitution of the active principle using techniques like colorimetry, spectrophotometery, flurometry etc.
It is the estimation of the biological activity in the unit quantity of the preparation of pharmacological molecule dosage. Biological systems like microorganism, isolated tissue, animals are employed in biological assays. The different methods for biological assays are matching the assay, interpolation and four point assays.
These assays employs diagnostic and quantitative method for assaying the drug. Radio-immuno assay is one of the widely used methods for some drugs and especially insulin in case of diabetes.
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