It is a highly sophisticated and technically demanding process. At a time, a fragment of 500-600 bp can be sequenced. Sequencing of genomes can be done by two methods:
a) Clone–By-Clone Sequencing: in this method the fragments are first aligned into contigs. It is also known as directed sequencing of BAC contigs.
b) Shot-Gun Sequencing: In this method randomly selected clones are sequenced until all clones in the genomic library are analyzed.
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