The replication of DNA is semi-conservative in nature which means that after the completion of replication, each DNA molecule would have one parental and one newly synthesized strand. The experimental proof that it replicates semi-conservatively was given by Messelson and stahl by their experiment on Escherichia coli using nitrogen and its isotopes. The main enzyme in replication is DNA-dependent DNA polymerase, as it uses DNA template to catalyze the polymerization of deoxynucleoside. The DNA-dependent DNA polymerase catalyses polymerization in one direction that is 5’-3’ direction. Replication originates from definite region known as origin of replication. In strand 3’-5 the replication is in continuous direction while on 5’-3’ end it is discontinuous. The discontinuously synthesized fragments are later joined by the enzyme DNA ligase. In eukaryotes, replication of DNA takes place at S-phase of cell cycle.
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