Laboratory Safety Introduction

Exercise 1

  1. Identify home substitutions for lab safety equipment. Record each item in step 1 into the column containing the most appropriate in-home substitution in Data Table 1.

Data Table 1: Lab Safety Equipment Alternatives

Lab Safety Equipment


Fire extinguisher

No Substitution

Spill containment kit

Paper Towels

Eyewash station

Shower or Sink

Safety goggles

No Substitution

Laboratory shower

Bathroom Shower

Fume hood

Well-Ventilated Area

First aid kit

No Substitution

Exercise 2

  1. Interpret how to read a Safety Data Sheet (SDS) by listing the basic information provided on a SDS.Record the SDS information for each of the items listed in step 2 into Data Table 2.

Data Table 2: Sodium Hypochlorite SDS information


SDS Information

Physical state

Liquid and no color

Precautionary Statement

1. Prevention

- Keep only in original container.

- Wash skin thoroughly after handling.

- Wear protective gloves/ protective clothing/ eye protection/ face protection.

2. Response

- IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.

- IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower.

- IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor.

- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor. Wash contaminated clothing before reuse. Absorb spillage to prevent material damage.

3. Storage

- Store locked up.

- Store in corrosive resistant container with a resistant inner liner.

4. Disposal

- Dispose of contents/ container to an approved waste disposal plant.

Required protective equipment

· Chemical splash goggles

· Protective gloves

· Appropriate protective clothing

· Respiratory protection program

First Aid Measure

1. General advice: First Aid responders should pay attention to self-protection and use the recommended protective clothing (chemical resistant gloves, splash protection). If potential for exposure exists refer to Section 8 for specific personal protective equipment.

2. Inhalation: Move person to fresh air; if effects occur, consult a physician.

3. Skin contact: Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing. Seek medical attention if symptoms occur or irritation persists. Wash clothing before reuse. Suitable emergency safety shower facility should be immediately available.

4. Eye contact: Wash eyes with plenty of water for 15 minutes at least. Do not forget to remove contact lenses. Suitable emergency eye wash facility should be immediately available.

5. Ingestion: Do not induce vomiting. Give one cup (8 ounces or 240 ml) of water or milk if available and transport to a medical facility. Do not give anything by mouth unless the person is fully conscious.

Fire-fighting measures

1. Suitable extinguishing media: In case of fire, use water fog, foam, dry powder, carbon dioxide.

2. Unsuitable extinguishing media: Do NOT use water jet. May spread fire. Dry chemical extinguishing agents may react with product; use with caution.

3. Special hazards arising from the substance or mixture Hazardous combustion products: During a fire, smoke may contain the original material in addition to combustion products of varying composition which may be toxic and/or irritating.

4. Advice for firefighters Fire Fighting Procedures: For safety reasons in case of fire, containers should be stored separately in closed containments. Do not breathe fumes.

5. Special protective equipment for firefighters: Wear full protective clothing and self-contained breathing apparatus.

Accidental Release Measure

1. Personal Precautions: General Hygiene Considerations: Wash contaminated clothing before reuse. Use personal protective equipment as required. Avoid contact with eyes and skin. Evacuate personnel to safe areas. Keep people away from and upwind of spill/leak.

2. Environmental Precautions: Prevent entry into waterways, sewers, basements or confined areas. Do not flush into surface water or sanitary sewer system. Prevent further leakage or spillage if safe to do so. Prevent product from entering drains.

3. Methods for Containment: Prevent further leakage or spillage if safe to do so.

4. Methods for Cleaning Up: Soak up with inert absorbent material. Pick up and transfer to properly labeled containers. After cleaning, flush away traces with water. Prevent product from entering drains. Dam up.

Safe storage

Keep container tightly closed. Store away from incompatible materials. See STABILITY AND REACTIVITY section. Store under cover in a dry, clean, cool, well ventilated place away from sunlight. Store away from oxidizing materials. Store in original vented container.

Safe disposal

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the Classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.

Environmental precautions and ecotoxicity

Very toxic to aquatic life with long lasting effects.

Spill cleanup procedures

Avoid runoff into storm sewers and ditches which lead to waterways. Clean up spills immediately, observing precautions in the Protective Equipment section. Absorb spill using an absorbent, noncombustible material such as earth, sand, or vermiculite. Do not use combustible materials such as sawdust. Provide ventilation.

Exercise 3

  1. What are small-scale techniques?

Small-scale techniques are a very small quantities of chemicals that contributed to the safety of the experiments. Small Scale Chemistry is an innovative, holistic, digital, user-friendly, transparent, quantitative, and sophisticated approach to engaging students in experimental chemistry. This provides a solution for most of the problems associated with laboratory instructions.

  1. List 5 precautions that must be taken before beginning an experiment.
  • Make sure you understand all the safety information provided to you before starting the experiment.
  • Review the related material safety data sheets (MSDS) for chemicals used (an example MSDS is shown). Complete the Emergency Contact Card and place it near your telephone (example emergency contact card is shown).
  • Always wear safety glasses (including when in and out). Contact lenses are prohibited and must also wear face shields. You must wear a lab coat, use closed shoes (no sandals, flat shoes, open footwear, etc.), wear long pants (no shorts, capris, skirts or dresses), for men or women who have long hair must Ponytail, and do not use excessive jewelry.
  • Make sure to wash your hands carefully before and after handling laboratory materials
  • Wear the safety gear (student shown putting on safety goggles, and safety gloves).
  1. As indicated in the video, what common substance can be used to neutralize a spilled acid?
  • Keep sodium bicarbonate, baking soda, on hand to neutralize the spilled assets before trying to wipe them (cat litter is used to absorb the spills shown).
  1. According to the video, why should a used chemical container never be refilled?
  • The chemicals used may be contaminated and can end in unexpected and potentially unpleasant reactions. Some chemicals include solvents. Most solvents are very flammable and should not be exposed to fire. Examples are alcohol, toluene, ether, acetone, and ethylacetate. Certain chemicals produce toxins that can be harmful if inhaled by us.
  1. Certain glass objects are not meant to be heated and could shatter if exposed to a heat source. What two examples of heat-sensitive glassware are given in the video?
  • The graduated cylinders and flasks are not intended to be heated and can be broken by heat sources. If a glass object breaks, do not risk cutting or lifting the glass with your hand. Instead, sweep the broken pieces and dispose of them quickly.

Exercise 4

  1. Review all content prior to this exercise until you can honestly state each listed safety statement. Then, you will sign the safety contract and print a safety card to fill-out. Record an “X” in Data Table 3 to agree or disagree with Part 1 and Record an “X” in Data Table 3 to agree or disagree with Part 2.

Data Table 3: Part 1 and Part 2 of the Safety Contract



Part 1:

It is not possible to control the use of HOL Science products, related devices, and student work environments. Author (s) content of HOL Science, instructors and institutions that adopt it, and Hands-On Labs, Inc. - publisher and producer of HOL Science - authorizing the use of this educational product has the condition that buyers and users accept full and complete responsibility for all and all responsibilities associated with their use. Please review this document several times until you are sure that you understand and will comply with the provisions that have been given in full.

I Agree.

Part 2:

I am a responsible adult who has read, understood and agreed to fully comply with all the precautions and safety measures specified in the HOL Science laboratory. For laboratory work and for the use of the HOL Science kit. Thus, I recognize the inherent dangers associated with scientific experiments. I will always experiment in a safe and wise way. I unconditionally accept full and complete responsibility for all responsibilities related to my purchase and / or use of the HOL Science scientific tool or science products or other materials provided by Hands-On Labs, Inc.

I Agree.