Use the data draw oxygen dissociation curve binding curve
Name: Due: 4:30 pm, April 5, 2021
Student Number: Homework 4
| pO2 (torr) | Y |
|---|---|
| 0 | 0 |
| 1 | 0.15 |
| 2 | 0.25 |
| 5 | 0.35 |
| 10 | 0.45 |
| 20 | 0.55 |
| 40 | 0.65 |
| 60 | 0.75 |
| 80 | 0.80 |
| 100 | 0.85 |
| 120 | 0.90 |
c. Based upon this graph, is there evidence for cooperativity in oxygen binding by this protein? Explain.
d. You run a sample of the isolated protein on gel filtration. You observe a single peak that elutes with a molecular weight of 108 kD. You also run SDS-PAGE and you observe a single band with an estimated molecular weight of 13.5 kD (either with or without β-mercaptoethanol). Based upon these results, what can you say about the quaternary structure of this molecule?
c. Hisβ146 → Alaβ146
3. Unlike humans, some mammals have identical subunit composition in adult and fetal hemoglobin. However, in those species, the concentration of 2,3-BPG is lower in the fetal circulation than in the maternal circulation. How would this difference in 2,3-BPG affect the transfer of oxygen from maternal to fetal circulation?
