Select the top degs with the largest absolute value log fold change

In order to study mouse early embyro development, RNA-seq experiments have been performed on mouse embyro at either zygote and 16-cell stages. Four replicates were used in each stage. The raw fastq files have been processed to count the reads in each gene from each library/sample. The Transcript Per Million (TPM) values were also calculated for each gene in each sample. In the links below, please download the read-count matrix file and TPM-matrix files and use DESeq2 to identify differentially expressed genes between zytoge and 16-cell embyro. For your convenience, the condition file is provided [ Download condition file ]. The following questions need to be answered:

1. How many genes in total have been studied by this RNA-seq experiments?

5. For DEGs found in question 3, if we require the fold-change of expression should be at least 8, how many DEGs are up-regulated and down-regulated respectively in 16-cell stage embyro?

6. Using the 16-cell stage up-regulated DEGs with at least 8-fold change to perform Gene ontology analysis on DAVID website, and report the significantly enriched Biological Process (BP) and Molecular Function (MF) terms (with FDR <= 0.05).