Effect of solvent exposure and temperature on beet cell membrane

Cells were exposed to temperatures ranging from -5C to 70C. Following incubation at the indicated temperature, water was added and the cells were allowed to incubate at room temperature for 20 minutes. Damage to cell membrane integrity was assessed by quantifying leakage of the pigment betacyanin via measuring light absorbance at 460 nm. [pic] Figure 2: The effect of solvent on cell membrane integrity. Cells were incubated in the presence of methanol, acetone, or saline at the indicated concentrations. Following incubation, damage to cell membrane integrity was assessed by quantifying leakage of the pigment betacyanin via measuring light absorbance at 460 nm.