Elegans life cycle and developmental stages

Caenorhabditis elegans Proposal

Jose Molina
Group Members: Arsal Ashraf, Celeon Mauricio, & Leah Miller Course Name: BIOL 3452 – 505

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Caenorhabditis elegans Proposal

Introduction

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experiment, and the gene which regulates this is him-8 (el1489) IV. Some other major gene classifications for C. elegans can be seen in Figure 3.

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Materials:

a NGM plate full of mutagenized C. elegans.

6. Then, after the F1 generation was allowed to lay eggs the adults were removed and flamed, and time was given for the eggs to hatch and develop.

7. Once the F2 generation has hatched, screen and observe for mutant worms.

Discussion & Conclusions

Based off our forward genetic screen, we hope to better understand the effect that the him-8 (e1489) IV gene has on the phenotypic behavior of C. elegans and how it creates a high incidence of males in the population since males only occur at a low frequency of about 0.05%.Observing the F2 generation will allow us to better understand the dominance of the gene, and will be able to see how often it is passed down. For future experiments the C. elegans should be allowed to feed on different non-pathogenic bacteria instead of E. coli in order to see observe the impact of a different diet on development. Also, a different mutagen could be used to cause different types of mutations. Using C. elegans to study genes can carry over to human diseases. Ultimately, understanding the genetic life cycle of C. elegans can be of great help in understanding more complex life cycles and lead us in the direction of better health care.

College of Biological Sciences. (2017, November 07). Retrieved November 08, 2017, from https://cbs.umn.edu/cgc/what-c-elegans

Erkut, C. (2014, July 08). C. elegans life cycle and developmental stages. Retrieved November 08, 2017, from
https://figshare.com/articles/C_elegans_life_cycle_and_developmental_stages/1096220