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# BIO lab 1408 microscopy and cytology report

MICROSCOPY AND CYTOLOGY REPORT SHEET

Bio Lab 1408

1. Microscopy

Experiment (dry lab). See the powerpoint slides to complete worksheet

1.  Locate the parts below and match them to their description.

objective lens stage

condenser lens with filter light

revolving nosepiece base

coarse adjustment knob rheostat

fine adjustment knob arm

iris diaphragm

 A Eyepiece ocular lens B Supports the ocular lenses head C Holds objectives that can be rotated revolving nosepiece D Connects the base and barrel arm E Lens that magnifies the specimen objective lens F Supports the slide stage G Focuses the light onto the specimen condenser lens with filter H Raises and lowers the stage for focusing coarse adjustment knob I Slightly moves the stage to sharpen the image fine adjustment knob J Illuminator light K Light control rheostat L Supports the microscope base M Varies the intensity of the light Iris diaphragm
1. Principles of microscopy

Experiment (dry lab)

Field of view

 Lens Lens Power Ocular lens Total Magnification Diameter in mm (3 significant figures) Diameter in mm (3 sig figs) Area in mm2 A = pr2 (p = 3.14) (3 sig figs) Scan Power 4X 10X 40X 4.200 4200 13.85 Low Power 10X 10x 100x 1.680 1680 22.16 High Power 40X 10x 400x 0.420 420 13.85 Oil Immersion 100X 10x 1000x0 0.168 168 26.73

Note:

• power of ocular lens does not change
• to determine total magnification use: lens power X ocular lens power
• to determine diameter in mm: 40x X 4.200/total mag = diameter in mm
• to convert mm to mm: mm X 1000 = mm
• to determine area: radius is ½ of diameter. Take radius and square it then multiply by p (pi)

What happens to the field of view as magnification increases?

 Decreases

(It increase, decrease or stay the same.)

-Depth of focus

What happens to the depth of focus as magnification increases?

 Decreases

(It increase, decrease or stay the same.).

-Image orientation

Select the letter ‘e’ as you would see through the ocular if it were mounted right side up on the slide. (A, B, C or D.)

 A

1. Cytology

Experiment (dry lab)

1. Wet Mounts
2. Eukaryotes
• Elodea leaf cells:
• Label the cell - plasma membrane, cell wall, chloroplast, and cytoplasm
 A Cell wall B Plasma membrane C Chloroplast D Cytoplasm
• Onion cells.
• Label the cell - plasma membrane, cell wall, cytoplasm, nucleolus, and nucleus
 A Cytoplasm B Cell membrane C Cell wall D Nucleolus E Nucleus

• Human cheek cells.
• Label the cell - plasma membrane, cytoplasm and nucleus
 A Plasma membrane B Nucleus C Cytoplasm
1. Prokaryotes

Identify these bacterial cells as coccus, bacillus or spirillum

 A Coccus B Bacillus C Spirillum
1. Cell Models: Label the animal and plant cells.

Animal cell model:

Label - centrioles (pair), cytoplasm, Golgi apparatus, mitochondria, nucleus and plasma membrane.

 Animal cell model #1 A Boundary between cytosol and extracellular fluid Plasma membrane B Flattened stacked discs Centrioles (pair) C Synthesize ATP Golgi apparatus D Cytosol + organelles Mitochondria E Found in pairs, made of microtubules Nucleus F Holds the DNA Cytoplasm

Nucleus:

Label - DNA (chromatin), nuclear membrane, nuclear pore and nucleolus.

 Animal cell/Plant cell Model #2 A Double membrane Nuclear pore B Assemble ribosomal subunits Nucleolus C Genetic material DNA (Chromatin) D Perforation on the nuclear membrane Nuclear membrane

Plant cell:

Label – cell wall, central vacuole, chloroplasts, cytoplasm, Golgi apparatus, plasma membrane, mitochondria and nucleus.

 Plant cell Model #3 A Boundary between cytosol and cell wall Plasma membrane B Synthesis of ATP Golgi apparatus C Flattened stacked discs Central vacuole D Supports the cell Cell wall E Holds the DNA Nucleus F Cytosol + organelles Mitochondria G Membranous sac stores water + other substances Cytoplasm H Site for photosynthesis Chloroplasts