Scatchard Analysis Assignment Help
Scatchard Analysis
A radio-labeled ligand (a hormone), is added at several concentration to a fixed amount of receptors and the fraction of the hormone bound to receptor is determined by separation the receptor/hormone complex from free hormone.
It is used to estimate the dissociation constant and the number of receptor binding sites in a given preparation.
The various techniques through which it is achieved are discussed in brief as follows:
- Spectroscopy
- Mass Spectrometry
- Chromatography
- Electrophoresis
- Isoelectric Focussing
- Microscopy
- Scatchard Analysis
- FRET
- Blotting
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