In this analytical method the analytes are first ionized in vacuum and then the charged molecules are introduced into an electric and also magnetic field , paths through the field is a function of their mass to charge ratio which is m/z. This measured property of the ionized species basically used to deduce the mass of the analytics with great precision. It provides a great deal of information for proteomics research, enzymology, and protein-chemistry. It is carried out by various methods:
a) Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) - In this, proteins are placed in a light-absorbing matrix, and then with a short pulse of laser light it is ionized, and then desorbed from the matrix into the vacuum system. It is used to measure the mass of wide range of macromolecules.
b) Electrospray ionization mass spectrometry (ESI-MS) - in this method the macromolecules in solution are forced directly from liquid to gas phase. Analytics solution is passed through a charged needle which is kept on a high electrical potential,which changed the solution into a fine mist charged droplets.
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