The construction of artificial recombinant DNA molecules is termed as genetic engineering or gene manipulation, sometimes also referred to as gene cloning or molecular cloning because it produces a line of genetically identical organisms, all of which contain the same recombinant DNA molecule.
The major steps involved in genetic engineering are:
The tools used for genetic engineering are those biological products or agents which are used to achieve its objectives. Four different types of enzymes are used viz. nucleases, ligases, polymerases, and certain DNA modifying enzymes.
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