It is the replacement of one gene function by another. The targeted gene transfer can be used to replace the normal endogenous gene with a specific mutant form of the same gene or the whole gene may be replaced by a DNA sequence desired.
A knockout mouse has one of its genes disrupted by the technique of targeted gene transfer; these mice permit the role of specific individual genes in development, in immune response studies and also provide animal models for evaluation of various therapeutic regiments.
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