Virtually all high value biochemicals from cultured plant cells are secondary metabolites, which are usually produced in differentiated cells or organized tissues. Therefore, most such biochemicals are not produced by rapidly cell cultures, and the culture conditions favoring growth suppress biochemical production. There is growth phase for cell biomass accumulation and production phase for biosynthesis and accumulation of biochemicals.
Biomass accumulation can be improved by using optimum culture conditions of which nutrient medium and inoculum size are particularly important.
Biomass production can be markedly increased by use of large inoculum size, to give higher initial cell density, in combination with proportionately enriched nutrient medium. e.g., twice the concentration of normal medium for a two-fold increased inoculum size.
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