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Dual Polarization Interferometry

The use of dual polarization interferometry to determining protein structures and their functions. As we all knows that proteins are the building blocks of life and most complex molecules, which is essential for all biological processes in body. Dual polarizations interferometry measures the protein structures in one dimension i.e it can measure the diameter or size, and density by the use of non-diffractive optics through coupling of the protein into a glass slide an probing its structure. This method reconciles the protein structures into the subatomic dimensions below 0.1 Angstrom.

DPI works on waveguides structures in which, light is not fully confined within physical boundaries on the guiding medium, but,rather decomposing exponentially at the boundaries. This decomposition gives changes in the refractive index of the material and then speed of propagation alter of the field. The advantages of DPI, probing the optical properties of proteins that it have been attached to the sensing surface by covalently, hydrophobically, and electrostatically.

It is detect the changing in optical path length through an optical field passing within the sensing path, which is governed by, other things, such as the interaction length and signal-to-noise ratio to the detection scheme. It is uses very simplified interferometry, which is based on slab waveguides, with reference arm buried within sensing arm. Then coherent light maximally illuminate stacks, both waveguides are traversing, and upon existing structures diffracts into free space. Diffracted wavefront generates Young's interference fringes in the far field, due to the waveguides and they are so close together. These changes in proteins molecules and interference patterns are captured by high resolution camera.

Measurement of interference pattern, can be determined by an optogeometric pattern for protein layer. But size or other structural signature will not be resolve unambiguously. Large protein molecules in smaller number density will produce same signals like in small protein in large number molecules.

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